Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (U2OS and RPE)
Total protein in input
3.5 µg
Specification
U2OS and RPE
Immuno-precipitation step
Protein A
Other product details
Dilution
1/200
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: NP40-buffer, 150mM NaCl
Western Blot antibody
Name
Non-Abcam antibody was used: anti-Rabbit IgG (Pierce)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Additional data
Additional Notes
lane 1: Input (2%), whole cell lysate from U2OS cells.
lane 2: Input (2%), whole cell lysate from RPE cells.
lane 3: control IP with IgG from whole cell lysate from U2OS cells.
lane 4: control IP with IgG from whole cell lysate from RPE cells.
lane 5: BRIT1 IP with ab121277 from whole cell lysate from U2OS cells.
lane 6: BRIT1 IP with ab121277 from whole cell lysate from RPE cells.
lane 2: Input (2%), whole cell lysate from RPE cells.
lane 3: control IP with IgG from whole cell lysate from U2OS cells.
lane 4: control IP with IgG from whole cell lysate from RPE cells.
lane 5: BRIT1 IP with ab121277 from whole cell lysate from U2OS cells.
lane 6: BRIT1 IP with ab121277 from whole cell lysate from RPE cells.
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投稿 Apr 16 2012