Application
Western blot
Sample
Zebrafish Cell lysate - other (fibroblast cell tipe)
Gel Running Conditions
Reduced Denaturing (SDS-PAGE 7.5%)
Loading amount
100 µg
Specification
fibroblast cell tipe
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: fat-free milk
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
LumiGLO cell signaling
Exposure
10 minute(s) and 0 second(s)
Positive control
NIH-3T3 fibroblast cellular lysate
Additional data
Additional Notes
this antibody do not work with zebrafish or ZF4 cell line lysates.
Abcam response
We have not tested this antibody with zebrafish samples so we were not sure if the antibody would cross react with this species. The antibody is validated and guaranteed for mouse, rat, and human. It did detect Brg1 in mouse samples (NIH-3T3 lysate).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Jorge Castillo
Verified customer
投稿 Mar 28 2016