Application
Western blot
Sample
Mouse Tissue lysate - whole (Ms Testis, Ms Lung, Ms Kidney, GC-1)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Ms Testis, Ms Lung, Ms Kidney, GC-1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2.5% Non-Fat Milk, PBS-Tween 0.1%
Concentration
1 µg/ml
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Swine anti-Rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Supersignal West Dura
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: 150 kDa Non-specific: 92, 34 (faint) kDa
Additional data
Additional Notes
Lane order (1-4): Ms Testis, Ms Lung, Ms Kidney, GC-1
This antibody detects a species of the predicted mobility in mouse lysates derived from testis extract (incl. GC-1 lysate) and lung extract. Weaker signal is observed in Kidney lysate. This expression pattern is consistent with array data published on the UCSC server (GNF Expression Atlas 2 Data from U133A and GNF1H Chips).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 12 2010