Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (osteosarcoma (u2os cells))
Total protein in input
40 µg
Specification
osteosarcoma (u2os cells)
Immuno-precipitation step
Protein A
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s)
Western Blot antibody
Name
Non-Abcam antibody was used: promega sheep anti-rabbit
Host species: Sheep
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Additional data
Additional Notes
First lane: marker (top is myosin (200kDa)
Second lane: untreated control IP
Third lane: IR-treated control IP
Fourth lane: untreated BRCA1 IP
Fifth lane: IR-treated BRCA1 IP
WB: BRCA1 (same antibody as IP)
Second lane: untreated control IP
Third lane: IR-treated control IP
Fourth lane: untreated BRCA1 IP
Fifth lane: IR-treated BRCA1 IP
WB: BRCA1 (same antibody as IP)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 06 2007