Application
Immunocytochemistry/ Immunofluorescence
Sample
African Green Monkey Cell (CV-1 (kidney))
Specification
CV-1 (kidney)
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton-X100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Other product details
Dilution
1/800
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 donkey anti-mous IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
ab16781 (1/800) detecting BRCA1 in 2Gy irradiated CV-1 cells in conjunction with a donkey anti-mouse secondary antibody conjugated to Alexa488 (green). Specify of the antibody was tested by discriminating between G1- and S/G2-phase cells by co-immunostaining with an antibody against CENP-F (red). Cells were also counterstained with DAPI in order to highlight the nucleus (blue).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Herr Dr. Gabor Rohaly
Verified customer
投稿 Jul 02 2012