Application
Western blot
Sample
Human Purified protein (Flag-tagged BRCA1)
Loading amount
0.1 µg
Specification
Flag-tagged BRCA1
Gel Running Conditions
Reduced Denaturing (6% gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1%BSA/TBST
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Mouse Secondary Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 220 kDa
Additional data
Additional Notes
Human Flag-tagged BRCA1 was overexpressed in HEK293 Cells and immunoprecipitated with anti-Flag antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 24 2010