Anti-BNP 抗体 (ab19645)
Key features and details
- Rabbit polyclonal to BNP
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-BNP antibody
BNP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BNP -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide corresponding to Human BNP conjugated to bovine thyroglobulin.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab19645の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/500. Detects a band of approximately 18 kDa (predicted molecular weight: 15 kDa).
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特記事項 |
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WB
1/500. Detects a band of approximately 18 kDa (predicted molecular weight: 15 kDa). |
ターゲット情報
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関連性
Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity. -
細胞内局在
Secreted -
参照データベース
- Entrez Gene: 4879 Human
- Entrez Gene: 25105 Rat
- Omim: 600295 Human
- SwissProt: P16860 Human
- SwissProt: P13205 Rat
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別名
- BNP(1-28) antibody
- BNP(1-29) antibody
- BNP(1-30) antibody
see all
画像
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All lanes : Anti-BNP antibody (ab19645) at 1/500 dilution
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 15 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 48 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (34)
ab19645 は 34 報の論文で使用されています。
- Heo JH et al. Letrozole Accelerates Metabolic Remodeling through Activation of Glycolysis in Cardiomyocytes: A Role beyond Hormone Regulation. Int J Mol Sci 23:N/A (2022). PubMed: 35008972
- Hu Y et al. Effects and Mechanism of Ganoderma lucidum Polysaccharides in the Treatment of Diabetic Nephropathy in Streptozotocin-Induced Diabetic Rats. Biomed Res Int 2022:4314415 (2022). PubMed: 35299891
- Yang Y et al. The lncRNA MIAT regulates CPT-1a mediated cardiac hypertrophy through m6A RNA methylation reading protein Ythdf2. Cell Death Discov 8:167 (2022). PubMed: 35383152
- Chen H et al. Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4. Clin Transl Med 12:e574 (2022). PubMed: 35390228
- Sun X et al. Paroxetine Attenuates Cardiac Hypertrophy Via Blocking GRK2 and ADRB1 Interaction in Hypertension. J Am Heart Assoc 10:e016364 (2021). PubMed: 33372534