Anti-BNIP3L/NIX 抗体 (ab8399)
Key features and details
- Rabbit polyclonal to BNIP3L/NIX
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-BNIP3L/NIX antibody
BNIP3L/NIX 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BNIP3L/NIX -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
-
ポジティブ・コントロール
- K562 cell lysatecan be used as positive control and the approximate 40kDa top band represents monomer of Bnip3L/NIX (4).
-
特記事項
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growingsubclass of the Bcl-2 family. A novel BH3 domaincontaining protein was recently identified and designated Bnip3L/NIX, Bnip3a, and Nix (for Nip3-like protein X) (Matsushima, M. et al.; Yasuda, M. et al.; Chen, G, et al.).Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Over expression of Bnip3L/NIX induces apoptosis (Yasuda, M. et al.; Chen, G, et al.). Bnip3L/NIX interacts withand overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L/NIX is localized in mitochondria. The messenger RNA of Bnip3L/NIX is ubiquitously expressed in human tissues (Matsushima, M. et al.; Yasuda, M. et al.). Bnip3L/NIX and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
-
精製度
Affinity purified -
一次抗体 備考
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growingsubclass of the Bcl-2 family. A novel BH3 domaincontaining protein was recently identified and designated Bnip3L, Bnip3a, and Nix (for Nip3-like protein X) (Matsushima, M. et al.; Yasuda, M. et al.; Chen, G, et al.).Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Over expression of Bnip3L induces apoptosis (Yasuda, M. et al.; Chen, G, et al.). Bnip3L interacts withand overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3Lis localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (Matsushima, M. et al.; Yasuda, M. et al.). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
軽鎖の種類
unknown -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8399の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40 kDa. To reduce possible background load less sample and or use more diluted antibody.
|
|
ICC/IF | (1) |
Use at an assay dependent concentration. See Abreview.
|
IHC-P |
Use a concentration of 2 µg/ml.
|
特記事項 |
---|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40 kDa. To reduce possible background load less sample and or use more diluted antibody. |
ICC/IF
Use at an assay dependent concentration. See Abreview. |
IHC-P
Use a concentration of 2 µg/ml. |
ターゲット情報
-
機能
Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. May function as a tumor suppressor. Inhibits apoptosis induced by BNIP3. -
配列類似性
Belongs to the NIP3 family. -
翻訳後修飾
Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin. -
細胞内局在
Nucleus envelope. Endoplasmic reticulum. Mitochondrion. Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 665 Human
- Entrez Gene: 12177 Mouse
- Entrez Gene: 140923 Rat
- Omim: 605368 Human
- SwissProt: O60238 Human
- SwissProt: Q9Z2F7 Mouse
- Unigene: 131226 Human
- Unigene: 29820 Mouse
see all -
別名
- Adenovirus E1B19k binding protein B5 antibody
- Adenovirus E1B19K-binding protein B5 antibody
- BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody
see all
画像
-
All lanes : Anti-BNIP3L/NIX antibody (ab8399) at 1 µg/ml
Lane 1 : K562 whole cell lysate with absence of immunogenic peptide
Lane 2 : K562 whole cell lysate with presence of immunogenic peptideThe approximate 40 kDa top band represents monomer of Bnip3L/NIX.
-
ab8399 at 2µg/ml staining BNIP3L/NIX in human kidney tissue by IHC
-
Immunofluorescence of Bnip3L/NIX in Human Kidney cells using ab8399 at 10 ug/ml.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (28)
ab8399 は 28 報の論文で使用されています。
- Shen Y et al. Methionine oxidation of CLK4 promotes the metabolic switch and redox homeostasis in esophageal carcinoma via inhibiting MITF selective autophagy. Clin Transl Med 12:e719 (2022). PubMed: 35092699
- Abdrakhmanov A et al. Receptor-Mediated Mitophagy Rescues Cancer Cells under Hypoxic Conditions. Cancers (Basel) 13:N/A (2021). PubMed: 34439183
- Zhu N et al. Berberine Protects Against Simulated Ischemia/Reperfusion Injury-Induced H9C2 Cardiomyocytes Apoptosis In Vitro and Myocardial Ischemia/Reperfusion-Induced Apoptosis In Vivo by Regulating the Mitophagy-Mediated HIF-1a/BNIP3 Pathway. Front Pharmacol 11:367 (2020). PubMed: 32292345
- Yu X et al. Hyperoxia exposure arrests alveolarization in neonatal rats via PTEN-induced putative kinase 1-Parkin and Nip3-like protein X-mediated mitophagy disorders. Int J Mol Med 46:2126-2136 (2020). PubMed: 33125104
- Hu Q et al. MicroRNA-137 exerts protective effects on hypoxia-induced cell injury by inhibiting autophagy/mitophagy and maintaining mitochondrial function in breast cancer stem-like cells. Oncol Rep 44:1627-1637 (2020). PubMed: 32945512