Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Testis
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/2000
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
For rating, I would rather use "Uncertain" than "Inconclusive" as I have no knowledge of the distribution pattern of this protein in tissue sections nor can I find published images.
Submitted image of adult testis shows a single large positive area in Sertoli cells ( black arrowhead) similar in developing sperm cells ( red arrowhead).
In the latter many cells show multiple positive dots ( green arrowhead).
In the Sertoli cells there is also a moderate diffuse cytoplasmic positivity.
Abcam response
Thank you for your valuable feedback. We were able to find evidence of BMP11 mRNA expression in the testis, but were unable to verify protein distribution in this tissue. We welcome further customer comments. For more information, please contact scientific support.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Jul 25 2013