Application
Western blot
Sample
Rat Tissue lysate - whole (kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
kidney
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5 % MP/TBST
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: HRP conjugated goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
20 second(s)
Bands
Specific: 55 kDa Non-specific: none kDa
Additional data
Additional Notes
One clear band was observed at approx 55 kDa, which is different from the reported 100 kDa for the long isoform of BMP1 for humans. Disc. Code 42962.
Abcam response
Thank you for your valuable feedback. We are sorry to hear that this antibody has not given you good results in WB using rat sample. This antibody has not been tested with this species before, and it is possible that it may not be suitable.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Sebastian Brings
Verified customer
投稿 Oct 21 2011