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ab4959 |
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ab3350 has been referenced in 8 publications.
Publishing research using ab3350? Please let us know so that we can cite the reference in this datasheet
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Anti-BMAL1 antibody - ChIP Grade (ab3350) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Predicted band size : 69.4 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 51 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
The 75 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to BMAL1.
ICC/IF image of ab3350 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3350, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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