Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - other (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-T with 5 % milk protein
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Bands
Specific: 45 kDa
Exposure
10 minute(s) and 0 second(s)
Positive control
Protein known from mass spect analysis
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Wayne Carter
Verified customer
投稿 Mar 28 2014