Application
Western blot
Sample
Human Cell lysate - whole cell (Endometrium (eutopic and ectopic))
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Specification
Endometrium (eutopic and ectopic)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST (0.1%)
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Rabbit IG, HRP-linked Whole Ab, Donkey
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Clarity Max Western ECL
Exposure
1 minute(s) and 20 second(s)
Bands
Specific: 30 kDa
Additional data
Additional Notes
Protein extracted using RIPA buffer (Thermo Scientific) with protease and phosphatase inhibitor cocktail (Thermo Scientific). Blot is low fluorescence PVDF membrane (BioRad).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 31 2017