Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/750
Secondary antibody
Dilution
1/200
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed
Additional data
Additional Notes
This antibody produces a strong, golgi-associated labeling pattern in both PF and MeOH fixed samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Sep 29 2016