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RabMAb

Anti-BCRP/ABCG2 抗体 [EPR2099(2)] (ab108312)

製品の概要

  • 製品名
    Anti-BCRP/ABCG2 antibody [EPR2099(2)]
    BCRP/ABCG2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2099(2)] to BCRP/ABCG2
  • アプリケーション
    適用あり: WBmore details
    適用なし: Flow Cyt,ICC,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in the intracellular domain of Human BCRP/ABCG2.

  • ポジティブ・コントロール
    • Human fetal liver, JAR, 293T, HepG2, and Human placenta lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • ターゲット情報

    • 機能
      Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
    • 組織特異性
      Highly expressed in placenta. Low expression in small intestine, liver and colon.
    • 配列類似性
      Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
      Contains 1 ABC transmembrane type-2 domain.
      Contains 1 ABC transporter domain.
    • 翻訳後修飾
      Glycosylation-deficient ABCG2 is normally expressed and functional.
    • 細胞内局在
      Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ABC transporter antibody
      • ABC15 antibody
      • ABCG 2 antibody
      • ABCG2 antibody
      • ABCG2_HUMAN antibody
      • ABCP antibody
      • ATP binding cassette sub family G (WHITE) member 2 antibody
      • ATP binding cassette transporter G2 antibody
      • ATP-binding cassette sub-family G member 2 antibody
      • BCRP antibody
      • BCRP1 antibody
      • BMDP antibody
      • Breast cancer resistance protein antibody
      • CD338 antibody
      • CDw338 antibody
      • CDw338 antigen antibody
      • EST157481 antibody
      • GOUT1 antibody
      • MGC102821 antibody
      • Mitoxantrone resistance associated protein antibody
      • Mitoxantrone resistance-associated protein antibody
      • MRX antibody
      • Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 antibody
      • MXR antibody
      • MXR1 antibody
      • Placenta specific ATP binding cassette transporter antibody
      • Placenta specific MDR protein antibody
      • Placenta-specific ATP-binding cassette transporter antibody
      • UAQTL1 antibody
      see all

    画像

    • All lanes : Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : JAR lysate
      Lane 3 : 293T lysate
      Lane 4 : HepG2 lysate
      Lane 5 : Human placenta lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 72 kDa

    プロトコール

    参考文献

    This product has been referenced in:
    • Pan X  et al. Downregulation of eIF4G by microRNA-503 enhances drug sensitivity of MCF-7/ADR cells through suppressing the expression of ABC transport proteins. Oncol Lett 13:4785-4793 (2017). WB . Read more (PubMed: 28599480) »
    • Zhan Y  et al. Hepatocellular carcinoma stem cell-like cells are enriched following low-dose 5-fluorouracil chemotherapy. Oncol Lett 12:2511-2516 (2016). Read more (PubMed: 27698820) »

    See all 3 Publications for this product

    レビューと Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (human liver)
    Permeabilization
    No
    Specification
    human liver
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    methanol:acetone (1:1 ratio)
    Username

    Abcam user community

    Verified customer

    投稿 Oct 30 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (ES-derived hepatocytes)
    Permeabilization
    Yes - BSA diluted in PBS/0.1% Tween
    Specification
    ES-derived hepatocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Methanol
    Username

    Abcam user community

    Verified customer

    投稿 Dec 12 2013

    Thank you for contacting us.

    For ab108312, you could use human fetal liver (ab29890), JAR, 293T (ab95494), HepG2 (ab7900) or human placenta (ab29745) lysates as a positive control.

    For ab32574, you could consider using MRP-1 trans...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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