Anti-BCRP/ABCG2 抗体 [1H2] (ab130244)
Key features and details
- Mouse monoclonal [1H2] to BCRP/ABCG2
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-BCRP/ABCG2 antibody [1H2]
BCRP/ABCG2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1H2] to BCRP/ABCG2 -
由来種
Mouse -
アプリケーション
適用あり: WB, ELISA, ICC/IFmore details -
種交差性
交差種: Mouse, Human, African green monkey -
免疫原
Purified recombinant fragment of Human BCRP/ABCG2 expressed in E. coli.
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ポジティブ・コントロール
- HepG2, COS7 and NIH/3T3 cell lysates; HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituent: 99.99% PBS -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
1H2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab130244の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 72 kDa.
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ELISA |
1/10000.
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ICC/IF |
1/200 - 1/1000.
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特記事項 |
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WB
1/500 - 1/2000. Predicted molecular weight: 72 kDa. |
ELISA
1/10000. |
ICC/IF
1/200 - 1/1000. |
ターゲット情報
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機能
Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123. -
組織特異性
Highly expressed in placenta. Low expression in small intestine, liver and colon. -
配列類似性
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain. -
翻訳後修飾
Glycosylation-deficient ABCG2 is normally expressed and functional. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9429 Human
- Entrez Gene: 26357 Mouse
- Omim: 603756 Human
- SwissProt: Q9UNQ0 Human
- SwissProt: Q7TMS5 Mouse
- Unigene: 480218 Human
- Unigene: 333096 Mouse
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別名
- ABC transporter antibody
- ABC15 antibody
- ABCG 2 antibody
see all
画像
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All lanes : Anti-BCRP/ABCG2 antibody [1H2] (ab130244) at 1/500 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : COS7 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : NIH/3T3 cell lysate
Predicted band size: 72 kDa -
ab130244, at 1/200 dilution, staining BCRP/ABCG2 in HeLa cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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ELISA using ab130244.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab130244 は 7 報の論文で使用されています。
- Yang H et al. FBI-1 enhanced the resistance of triple-negative breast cancer cells to chemotherapeutic agents via the miR-30c/PXR axis. Cell Death Dis 11:851 (2020). PubMed: 33051436
- Ke B et al. Interleukin-7 Resensitizes Non-Small-Cell Lung Cancer to Cisplatin via Inhibition of ABCG2. Mediators Inflamm 2019:7241418 (2019). PubMed: 31915416
- Cheng CC et al. Stat3/Oct-4/c-Myc signal circuit for regulating stemness-mediated doxorubicin resistance of triple-negative breast cancer cells and inhibitory effects of WP1066. Int J Oncol 53:339-348 (2018). PubMed: 29750424
- Wang P et al. HIF1a regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia. Oncotarget 8:28074-28092 (2017). PubMed: 28427209
- Park SJ et al. Estradiol, TGF-ß1 and hypoxia promote breast cancer stemness and EMT-mediated breast cancer migration. Oncol Lett 11:1895-1902 (2016). PubMed: 26998096
- Topalis D et al. Resistance to the nucleotide analogue cidofovir in HPV(+) cells: a multifactorial process involving UMP/CMP kinase 1. Oncotarget 7:10386-401 (2016). WB . PubMed: 26824416
- Wang C et al. Associations between ABCG2 gene polymorphisms and isolated septal defects in a Han Chinese population. DNA Cell Biol 33:689-98 (2014). PubMed: 24979295