Anti-BAP31 抗体 [7A3BB6] (ab112993)
Key features and details
- Mouse monoclonal [7A3BB6] to BAP31
- Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-BAP31 antibody [7A3BB6]
BAP31 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7A3BB6] to BAP31 -
由来種
Mouse -
特異性
Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques. -
アプリケーション
適用あり: IHC-P, ICC/IF, WB, Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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ポジティブ・コントロール
- WB: HEK293T, HeLa, NCCIT and HepG2 cell lysates. Flow Cyt: HL60 cells. ICC/IF: Human fibroblast cells. IHC-P: Human normal colon FFPE tissue.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
ab112993 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%). -
ポリ/モノ
モノクローナル -
クローン名
7A3BB6 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab112993の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 µg/ml.
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 28 kDa.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. |
ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 28 kDa. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis. -
組織特異性
Ubiquitous. -
関連疾患
Note=BCAP31 is deleted in the chromosome Xq28 deletion syndrome which involves BCAP31 and the and the promoter region of ABCD1. -
配列類似性
Belongs to the BCAP29/BCAP31 family. -
翻訳後修飾
Cleaved by CASP8 and other caspases. -
細胞内局在
Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. May shuttle between the ER and the intermediate compartment/cis-Golgi complex. - Information by UniProt
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参照データベース
- Entrez Gene: 10134 Human
- Entrez Gene: 27061 Mouse
- Entrez Gene: 293852 Rat
- Omim: 300398 Human
- SwissProt: P51572 Human
- SwissProt: Q61335 Mouse
- Unigene: 522817 Human
- Unigene: 17 Mouse
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別名
- 6C6 AG antibody
- 6C6 AG tumor associated antigen antibody
- 6C6-AG tumor-associated antigen antibody
see all
画像
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All lanes : Anti-BAP31 antibody [7A3BB6] (ab112993) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : BCAP31 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : NCCIT cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaLanes 1-4: Merged signal (red and green). Green - ab112993 observed at 28 kDa. Red - loading control ab52901 observed at kDa.
ab112993 Anti-BAP31 antibody [7A3BB6] was shown to specifically react with BAP31 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266634 (knockout cell lysate ab257857) was used. Wild-type and BAP31 knockout samples were subjected to SDS-PAGE. ab112993 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (ab52901) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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ab112993 at 1ug/ml staining BAP31 in MeOH-fixed HL60 cells by Flow Cytometry (blue). Isotype control antibody (red).
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ab112993 at 5 µg/ml staining BAP31 in Human fibroblast cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green). The target locates mainly in the endoplasmic reticulum.
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IHC image of BAP31 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112993, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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All lanes : Anti-BAP31 antibody [7A3BB6] (ab112993) at 0.5 µg/ml
Lane 1 : HepG2 Cell Lysate
Lane 2 : H9C2 Cell Lysate
Lane 3 : MEF Cell Lysate
Predicted band size: 28 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab112993 は論文での使用が確認できていません。