Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole adult brain lysate)
Specification
Whole adult brain lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 30 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Jackson donkey anti goat
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 60 kDa Non-specific: 50, 80 kDa
Additional data
Additional Notes
The specific band is the most prominent on the blot. Left lane is ladder, right lane is whole brain extract.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 08 2006