Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela cells)
Specification
Hela cells
Fixative
Methanol
Permeabilization
No
Blocking step
Fish scale gelatin in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Other product details
Dilution
1/300
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: 0.2 % Fish scale gelatin in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
B MyB protein is clearly visualized (green channel) in the nuclear of Hela cells by immunostaining using B MyB antibody (ab12296)
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投稿 Aug 02 2010