Application
Immunocytochemistry/ Immunofluorescence
Sample
African Green Monkey Cell (CV1)
Specification
CV1
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Dilution
1/400
Additional data
Additional Notes
CV1 cells were fixed in 4% paraformaldehyde for 30 min at 4C. cells were blocked with 5% FCS in PBS for 1 hr at RT and incubated with anti-Axl diluted 1:100 in PBS over night at 4C. DAPI was used to counterstain nuclei.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Nir Drayman
Verified customer
投稿 Apr 21 2011