Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH 3T3)
Permeabilization
No
Specification
NIH 3T3
Fixative
Methanol
Other product details
Incubation time
30 minute(s) · Temperature: 37°C · Diluent: 1x PBS Tween-20
Dilution
1/500
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Texas Red Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Texas Red ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Texas Red ®
Additional data
Additional Notes
We have tested this antibody in NIH-3T3, CHO, and RPE cells. Attached are the resulting images of these stains. Aurora A is successfully stained, however, Aurora B is stained as well. This antibody is cross-reactive with these species, however is also cross-reactive with Aurora B in these cells. All cell types were fixed with methanol. Staining was successful with 1/500 dilutions as well as 1/1000. Increasing the dilution does help to weaken the Aurora B staining.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 14 2015