Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Mouse Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
This antibody only recognizes a centrosome associated epitope in methanol-fixed cells and does not recognize anything in a paraformaldehyde-fixed cell. The mitotic signal is extremely bright and a dilution of 1:2000 was used. The interphase centrosome (pre/post centrosome duplication) was observed at a dilution of 1:200
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Apr 19 2007