製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Ataxin 7
  • アプリケーション
    適用あり: ICC/IF, ICC, IP, IHC-Fr, WBmore details
  • 種交差性
    交差種: Human, Drosophila melanogaster
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human Ataxin 7 aa 1-17.
    Sequence:

    MSERAADDVRGEPRRAA


    (Peptide available as ab39761)

  • ポジティブ・コントロール
    • Whole cell lysate from COS-1 cells transfected with Ataxin 7.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
ICC 1/500.
IP Use at an assay dependent concentration.
IHC-Fr 1/500 - 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 96 kDa.Can be blocked with Human Ataxin 7 peptide (ab39761).

ターゲット情報

  • 関連性
    Ataxin 7 is a protein of unknown function. It may be the human orthologue of the yeast SAGA SGF73 subunit and a subunit of the human TFTC-like transcriptional complexes. Spinocerebellar ataxia 7 (one of a group of hereditary neurodegenrative diseases) is caused by an expanded trinucleotide repeat in the gene encoding ataxin 7. Ataxin 7 is typically located in the cytoplasm and on the nuclear membrane of normal brain neurons. In cells where there is a mutation of the SCA7 gene, ataxin 7 accumulates in intranuclear inclusions and can result in cell death.
  • 細胞内局在
    Cytoplasmic (isoform b) and Nuclear (isoform a)
  • 参照データベース
  • 別名
    • ADCAII antibody
    • ATXN 7 antibody
    • OPCA III antibody
    • OPCA3 antibody
    • SCA 7 antibody
    • SCA7 antibody
    • Spinocerebellar Ataxia 7 antibody
    • Spinocerebellar ataxia type 7 protein antibody
    see all

画像

  • Western blot of Ataxin 7 in transfected COS-1 cell lysate using ab11434.
  • ICC/IF image of ab11434 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11434, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab11434 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録