Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100 in PBS
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Additional Notes
Asynchronous HeLa were either paraformaldehyde or methanol fixed, permeablized and immunofluorescently labeled. Cells were counterstained with DAPI. The PF fixed samples produced a very weak nuclear signalwith a high degree of background following long exposure times. MeOH fixation does not appear to be compatible with this antibody as no nuclear enrichment was observed.
Abcam response
Thank you for your valuable feedback. ICC/IF was not a tested application for ab72111 and it seems that this antibody does not recognize Atad5 under these experimental conditions. Thank you for your time - we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Feb 11 2009