Application
Western blot
Sample
Human Cell lysate - whole cell (HL60)
Loading amount
50 µg
Specification
HL60
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk TBS
Secondary antibody
Name
Non-Abcam antibody was used: ECL Rabbit IgG, HRP-Linked F(ab')2 Fragment
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 minute(s) and 0 second(s)
Additional data
Additional Notes
Repeated the experiment a couple of times. Same result.
Abcam response
We suggest a positivive control of K562 cells which constituitively express high levels of asparagine synthetase. As noted in the Relevance section of our datasheet, certain tumor cells exhibit little or no asparagine synthetase activity. We do not have data for HL60 but at least one other leukemia, ALL (acute lymphoblastic) has been treated with asparaginase to take advantage of this deficiency.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Jul 09 2007