Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
(agent) for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate, pH6
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Blocking buffer
Dilution
1/1500
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Mice treated with E.coli Asparaginase or mock treated mice were sacrificed 16 hours after injection. Tissue was stained for Asparaginase uptake.
This antibody works nicely but quality of detection is a bit variable.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 31 2014