Anti-Asialoglycoprotein Receptor 1/HL-1 抗体 (ab88042)
Key features and details
- Rabbit polyclonal to Asialoglycoprotein Receptor 1/HL-1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Asialoglycoprotein Receptor 1/HL-1 antibody
Asialoglycoprotein Receptor 1/HL-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Asialoglycoprotein Receptor 1/HL-1 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab254261.
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アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab98248) -
ポジティブ・コントロール
- This antibody gave a positive signal in HepG2 whole cell lysate and FFPE human liver tissue sections.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88042の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 33 kDa).
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特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 33 kDa). |
ターゲット情報
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機能
Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface. -
組織特異性
Expressed exclusively in hepatic parenchymal cells. -
配列類似性
Contains 1 C-type lectin domain. -
翻訳後修飾
Phosphorylated on a cytoplasmic Ser residue. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 432 Human
- Entrez Gene: 100172364 Orangutan
- Omim: 108360 Human
- SwissProt: P07306 Human
- SwissProt: Q5RBQ8 Orangutan
- Unigene: 12056 Human
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別名
- ASGP-R 1 antibody
- ASGPR 1 antibody
- ASGPR antibody
see all
画像
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All lanes : Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab88042) at 1 µg/ml
Lane 1 : Human Liver Tissue Lysate
Lane 2 : HepG2 Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 44,48 kDa why is the actual band size different from the predicted?
Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk/TBST before being incubated with ab88042 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
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Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab88042) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThe predicted molecular weight of Human Asialoglycoprotein Receptor 1 / HL-1 is 33 kDa (SwissProt), however we expect to observe a banding pattern at 46 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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IHC image of ab88042 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88042, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab88042 は 5 報の論文で使用されています。
- Mancio-Silva L et al. Improving Drug Discovery by Nucleic Acid Delivery in Engineered Human Microlivers. Cell Metab 29:727-735.e3 (2019). PubMed: 30840913
- Workinger JL et al. Systemically Administered Plant Recombinant Holo-Intrinsic Factor Targets the Liver and is not Affected by Endogenous B12 levels. Sci Rep 9:12269 (2019). PubMed: 31439908
- Casey CA et al. Giantin Is Required for Post-Alcohol Recovery of Golgi in Liver Cells. Biomolecules 8:N/A (2018). PubMed: 30453527
- Zhang D et al. Simplified quantification method for in vivo SPECT/CT imaging of asialoglycoprotein receptor with (99m)Tc-p(VLA-co-VNI) to assess and stage hepatic fibrosis in mice. Sci Rep 6:25377 (2016). PubMed: 27150943
- Casey CA et al. Study of Ethanol-Induced Golgi Disorganization Reveals the Potential Mechanism of Alcohol-Impaired N-Glycosylation. Alcohol Clin Exp Res 40:2573-2590 (2016). PubMed: 27748959