Application
Western blot
Sample
Human Tissue lysate - whole (Pooled endometrial tissue)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Pooled endometrial tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST (0.1% Tween)
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: GE NA934-1ML Rabbit IG, HRP-linked Whole Ab
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Clarity Max Western ECL
Negative control
Lane 4
Exposure
7 second(s)
Bands
Specific: 50 kDa Non-specific: 25 kDa
Additional data
Additional Notes
Protein extracted using RIPA buffer (Thermo Scientific) with protease and phosphatase inhibitor cocktail (Thermo Scientific). Blot is low fluorescence PVDF membrane (BioRad). Lane 1=20 ug, Lane 2 = 10 ug, Lane 3 = 5 ug, Lane 4 = 0 ug
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 30 2017