Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample
Mouse Cell (Mouse Ovarian Tumor)
Specification
Mouse Ovarian Tumor
Permeabilization
Yes - 0.1% Triton X
Fixative
NBF
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Dilution
1/500
Additional data
Additional Notes
This antibody has also been utilized for aromatase detection in mouse ovarian tumor tissues (fig). IHC was carried out using HIER procedure with 0.1M citrate buffer at pH 6.0 and AEC for staining. Antibody concentration was same as ICC.
PubMed ID
DOI: 10.1371/journal.pgen.1004230: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kannan Athilakshmi
Verified customer
投稿 Mar 10 2014