Application
Immunohistochemistry (Frozen sections)
Sample
Dog Tissue sections (ovary)
Specification
ovary
Fixative
Acetone
Permeabilization
Yes - acetone 10 min
Blocking step
1%BSA, 10%NGS in TBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/50
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1%BSA in TBS
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Dilution
1/100
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. David Matlack
Verified customer
投稿 Dec 17 2008