Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (spleen)
Specification
spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization
No
Blocking step
Sequential peroxidase (5min) and protein (10min) block (prediluted). Reagents from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Other product details
Dilution
1/50
Incubation time
40 minute(s) · Temperature: 20°C · Diluent: Antibody diluent
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
Stained specific cells - these are marked by arrows. This staining was not present in the negative control. OVernight at 4oC would probably reduce the slight background and allow more dilute primary to be used. Secondary reagents from Dako
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
Verified customer
投稿 Aug 19 2008