Anti-Argininosuccinate Lyase 抗体 (ab97370)
Key features and details
- Rabbit polyclonal to Argininosuccinate Lyase
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Argininosuccinate Lyase antibody
Argininosuccinate Lyase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Argininosuccinate Lyase -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow -
免疫原
Recombinant fragment corresponding to Human Argininosuccinate Lyase aa 13-231.
Sequence:FVGAVDPIMEKFNASIAYDRHLWEVDVQGSKAYSRGLEKAGLLTKAEMDQ ILHGLDKVAEEWAQGTFKLNSNDEDIHTANERRLKELIGATAGKLHTGRS RNDQVVTDLRLWMRQTCSTLSGLLWELIRTMVDRAEAERDVLFPGYTHLQ RAQPIRWSHWILSHAVALTRDSERLLEVRKRINVLPLGSGAIAGNPLGVD RELLRAELNFGAITLNSMDAPEATLGPCWGCSCGSDPGVGRAGLGGRPVA LLYRCCFCGEDHPRQGSILYSLLTSSKQTHVAPAAPEARPGGAWWDRSYF AQRPGGKEALPGGRATALLYRCCFCGEDHPQQGSTLYCVPTSTNQAQAAP EERPRAPWWDTSSGALRPVALKSPQVVCEAASAGLLKTLRFVKYLPCFQV LPLDQQLVLVRNCWASLLMLELAQDRLQFETVEVSEPSMLQKILTTRRRE TGGNEPLPVPTLQHHLAPPAEARKVPSASQVQAIKCFLSKCWSLNISTK
Database link: P04424 -
ポジティブ・コントロール
- WB: HepG2. HEK-293T, HeLa, A431 whole cell lysate. Neuro2a, GL261, C8D30, NIH/3T3, BCL-1, Raw 264.7, C2C12 whole cell lysate. ICC/IF: HeLa cells IHC-P: U-87 MG xenograft, C2C12 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97370の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
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ICC/IF |
1/100 - 1/200.
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IHC-P |
1/100 - 1/1000.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa. |
ICC/IF
1/100 - 1/200. |
IHC-P
1/100 - 1/1000. |
ターゲット情報
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パスウェイ
Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.
Nitrogen metabolism; urea cycle; L-arginine and fumarate from (N(omega)-L-arginino)succinate: step 1/1. -
関連疾患
Defects in ASL are the cause of arginosuccinicaciduria (ARGINSA) [MIM:207900]. Arginosuccinicaciduria is an autosomal recessive disorder of the urea cycle. The disease is characterized by mental and physical retardation, liver enlargement, skin lesions, dry and brittle hair showing trichorrhexis nodosa microscopically and fluorescing red, convulsions, and episodic unconsciousness. -
配列類似性
Belongs to the lyase 1 family. Argininosuccinate lyase subfamily. -
翻訳後修飾
Acetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TSA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity. - Information by UniProt
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参照データベース
- Entrez Gene: 435 Human
- Entrez Gene: 109900 Mouse
- Entrez Gene: 59085 Rat
- Omim: 608310 Human
- SwissProt: P04424 Human
- SwissProt: Q91YI0 Mouse
- SwissProt: P20673 Rat
- Unigene: 632015 Human
see all -
別名
- Argininosuccinase antibody
- Argininosuccinate lyase antibody
- Arginosuccinase antibody
see all
画像
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Paraffin embedded C2C12 xenograft tissue stained for ASL using ab97370 at 1/500 dilution in immunohistochemical analysis.
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HeLa cells stained for ASL (green) using ab97370 at 1/500 dilution in ICC/IF.
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All lanes : Anti-Argininosuccinate Lyase antibody (ab97370) at 1/1000 dilution
Lane 1 : Neuro2a whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lane 4 : NIH/3T3 whole cell lysate
Lane 5 : BCL-1 whole cell lysate
Lane 6 : Raw 264.7 whole cell lysate
Lane 7 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa10% SDS-PAGE.
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Paraffin embedded U-87 MG xenograft tissue stained for ASL using ab97370 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-Argininosuccinate Lyase antibody (ab97370) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa10% SDS-PAGE.
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ab97370, at a 1/200 dilution, staining Argininosuccinate Lyase in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab97370 は 7 報の論文で使用されています。
- Zhang W et al. The related mechanism of complete Freund's adjuvant-induced chronic inflammation pain based on metabolomics analysis. Biomed Chromatogr 35:e5020 (2021). PubMed: 33159321
- Lerner S et al. ASL expression in ALDH1A1+ neurons in the substantia nigra metabolically contributes to neurodegenerative phenotype. Hum Genet 140:1471-1485 (2021). PubMed: 34417872
- Drokhlyansky E et al. The Human and Mouse Enteric Nervous System at Single-Cell Resolution. Cell 182:1606-1622.e23 (2020). PubMed: 32888429
- Lerner S et al. ASL Metabolically Regulates Tyrosine Hydroxylase in the Nucleus Locus Coeruleus. Cell Rep 29:2144-2153.e7 (2019). PubMed: 31747589
- Lee JS et al. Urea Cycle Dysregulation Generates Clinically Relevant Genomic and Biochemical Signatures. Cell 174:1559-1570.e22 (2018). PubMed: 30100185
- Baruteau J et al. Argininosuccinic aciduria fosters neuronal nitrosative stress reversed by Asl gene transfer. Nat Commun 9:3505 (2018). PubMed: 30158522
- Marini JC et al. The intestinal-renal axis for arginine synthesis is present and functional in the neonatal pig. Am J Physiol Endocrinol Metab 313:E233-E242 (2017). PubMed: 28611027