Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris NuPAGE)
Loading amount
40 µg
Specification
Lung
Blocking step
Odyssey TBS Blocking Buffer (proprietary) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Other product details
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 50% Odyssey TBS Blocking Buffer+Tween
Dilution
1/5000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye CW800
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Near-infrared
Negative control
Primary antibody omitted, which resulted in no bands (not shown).
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 25 kDa Non-specific: 130, 250 kDa
Positive control
Same tissue lysate was used with an anti-beta-actin antibody from Abcam, known to produce good signal. Image not shown.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Pete Serbedzija
Verified customer
投稿 May 25 2016