Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (AQP5-EGFP transfected cells / rat lung slices)
Permeabilization
Yes - 0,2 % Saponin
Specification
AQP5-EGFP transfected cells / rat lung slices
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: Room temperature
Dilution
1/200
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Secondary Antibody, A
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
A) HEK cells were transfected with AQP5 (cDNA from rat lung) C-terminal tagged to EGFP.
B) PFA-fixed slices from rat lung were stained with DAPI, ab92320 (red) and ab24751 (cyan)
Abcam response
Thank you for participating in our Abtrial program and your valuable feedback. We are pleased that this antibody worked well for you in rat, a species that has not been tested before. We welcome any further feedback and Abreviews from other customers who have used this antibody in rat which will help us assess how well the antibody works in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 01 2015