製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Aquaporin 3
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IF, IP, Flow Cyt, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Aquaporin 3 aa 278-292 (C terminal).
    Sequence:

    EENVKLAHVKHKEQI


    (Peptide available as ab195690)

  • ポジティブ・コントロール
    • WB: Rat kidney, mouse lung and mouse kidney tissue lysates. IHC-P: Rat kidney tissue. ICC/IF: formaldehyde fixed MCF-7 cells.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride, 2.5% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab125219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.Can be blocked with Aquaporin 3 peptide (ab195690). The detection limit of ab125219 is approximately 1ng/lane under non-reducing and reducing conditions.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
IP Use a concentration of 5 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.

ターゲット情報

  • 機能
    Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.
  • 組織特異性
    Widely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level.
  • 配列類似性
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • ドメイン
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • 細胞内局在
    Basolateral cell membrane. In collecting ducts of kidney.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AQP 3 antibody
    • AQP-3 antibody
    • Aqp3 antibody
    • AQP3_HUMAN antibody
    • Aquaglyceroporin-3 antibody
    • Aquaporin 3 (GIL blood group) antibody
    • Aquaporin 3 (Gill blood group) antibody
    • Aquaporin-3 antibody
    • Aquaporin3 antibody
    • GIL antibody
    • Gill blood group antibody
    see all

画像

  • All lanes : Anti-Aquaporin 3 antibody (ab125219) at 0.5 µg/ml

    Lane 1 : Rat Kidney Tissue Lysate at 50 µg
    Lane 2 : Rat Lung Tissue Lysate at 50 µg
    Lane 3 : Mouse Kidney Tissue Lysate at 50 µg
    Lane 4 : MM453 Whole Cell Lysate at 40 µg
    Lane 5 : SMMC-7721 Whole Cell Lysate at 40 µg


    Predicted band size : 31 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • ab125219 at 1µg/ml staining Aquaporin 3 in Paraaffin-embedded Rat kidney tissue by Immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Lung Cancer Tissue labeling Aquaporin 3 with ab125219.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Renal Cancer Tissue labeling Aquaporin 3 with ab125219.

  • Immunohistochemistry (Frozen sections) analysis of Rat Kidney Tissue labeling Aquaporin 3 with ab125219.

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Aquaporin 3 with ab125219.

  • All lanes : Anti-Aquaporin 3 antibody (ab125219) at 1 µg/ml

    Lane 1 : Rat kidney tissue lysate
    Lane 2 : Mouse lung tissue lysate
    Lane 3 : Mouse kidney tissue lysate


    Predicted band size : 31 kDa
  • Aquaporin 3 was immunoprecipitated using 0.5mg Mouse Kidney extract, 5µg of Rabbit polyclonal to Aquaporin 3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Kidney extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab125219.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 31kDa: Aquaporin 3.
  • ICC/IF image of ab125219 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab125219 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Na TY  et al. The trisaccharide raffinose modulates epidermal differentiation through activation of liver X receptor. Sci Rep 7:43823 (2017). WB, ICC ; Mouse, Human . Read more (PubMed: 28266648) »
  • Bakry OA  et al. Aquaporin 3 and E-Cadherin Expression in Perilesional Vitiligo Skin. J Clin Diagn Res 10:WC01-WC06 (2016). Read more (PubMed: 28208984) »

See all 6 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Intervertebral Disc)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: 1X TBS, 0.1% Calcium Chloride/alpha-Chymotrypsin
Permeabilization
No
Specification
Intervertebral Disc
Blocking step
1% BSA in 75% TBS 25% Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 25% · Temperature: 18°C
Fixative
10% Neutral Buffered Formalin
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投稿 Jul 14 2017

Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
No
Sample
Human Cell (Human bronchial epithelial cells)
Specification
Human bronchial epithelial cells
Gating Strategy
Single cells (gated on FSC-H vs FSC-W), debris excluded (gated on FSC-A vs SSC-A) and live cells (gated on live/dead stain negative population)
Preparation
Cell harvesting/tissue preparation method: Trypsinisation
Sample buffer: 10% DMEM
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投稿 Apr 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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