Application
Western blot
Sample
Mouse Cell lysate - whole cell (IMCD Cells)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
25 µg
Treatment
- and + 500 ´M dbcAMP
Specification
IMCD Cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: POD-F (ab)2-anti-Rabbit-IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Donkey
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 25, 37 kDa Non-specific: 60, 75 kDa
Positive control
with N6,2'-O-Dibutyryladenosine 3',5'-cyclic monophosphate sodium salt (dbcAMP) stimulation 500 ´M for 1 h
Additional data
Additional Notes
The IMCD cells are published in this paper:
Culturing Primary Rat Inner Medullary Collecting Duct Cells
Faust D, Geelhaar A, Eisermann B, Eichhorst J, Wiesner B, Rosenthal W, Klussmann E. J Vis Exp. 2013 Jun 21(76). doi: 10.3791/50366.
PubMed ID
23852264: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Andrea Geelhaar
Verified customer
投稿 May 30 2017