Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.1 M PBS with 1% Triton X
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 0.1M PBST with 10%donkeys serum
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa fluoro 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Three months old APP transgenic mouse was perfused with 4% PFA in 0.1M PBS. IFC staining performed in brain sections with rabbit MAB anti- ApoE-Ab52607 with Alexa488 and astrocytic marler GFAP-568. ApoE expressed in the ventricular wall and co-localised with GFAP-568. Nucleus stained with DAPI.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
投稿 Feb 05 2016