Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Cell (Brain section)
Specification
Brain section
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Other product details
Dilution
1/500
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% TritonX with 0.1x PBS- 10% Donkeys
Secondary antibody
Name
Non-Abcam antibody was used: Anti Rabbit Alexa Fluro 568
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Dilution
1/1000
Additional data
Additional Notes
Four months old APP transgenic mouse was perfused with 4% PFA in 0.1M PBS. IFC staining performed in brain sections with anti-Apolipoprotein E (ab1907) with Alexa568. APOE express in the choroid plexus of mouse brain. Nucleus stained with DAPI.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
投稿 Aug 07 2012