Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Liver)
Permeabilization
Yes - 0.1 M PBS with 3% Triton X
Specification
Liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.3M PBST with 10%donkeys serum
Dilution
1/500
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa fluoro 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Three months old ApoE knock out (KO) mouse was perfused with 4% PFA in 0.1M PBS. IFC staining performed in liver sections with rabbit MAB ApoCIII-Ab76305 and secondary antibody Alexa488. ApoCIII expressed in the hepatocytes. In ApoC-KO mice a large numbers of abnormal hepatocytes are visible.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
投稿 Feb 05 2016