Application
Flow Cytometry
Sample
Human Cell (U266 cell line B lymphocyte (myeloma))
Permeabilization
No
Gating Strategy
total stained cells
Specification
U266 cell line B lymphocyte (myeloma)
Preparation
Cell harvesting/tissue preparation method: suspension cells, so used directly
Sample buffer: RPMI 1640 medium with 2 mM L-glutamine
Sample buffer: RPMI 1640 medium with 2 mM L-glutamine
Fixation
Unfixed
Other product details
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: FACS buffer (PBS with 0.1% azide)
Dilution
1/60
Secondary antibody
Concentration
1 µg/ml
Name
Non-Abcam antibody was used: anti-rabbitFITC
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Additional data
Additional Notes
No binding seen with FACS in this cell line. This cell line is part of our work where ApoC3 is a potential marker in myeloma tissue extracts.
Abcam response
Thank you for your honest and valuable feedback. Your data suggest ab76305 may not be suitable for flow cytometry under the experimental conditions in which you tested. To confirm this finding, have you considered including a positive control like human liver tissue lysate listed on the data sheet? Thank you for your time - we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Prof. Paul Townsend
Verified customer
投稿 May 07 2010