Anti-ATG3 抗体 [7A1D1] (ab233560)
Key features and details
- Mouse monoclonal [7A1D1] to ATG3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
製品の概要
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製品名
Anti-ATG3 antibody [7A1D1]
ATG3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7A1D1] to ATG3 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human, African green monkey
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Recombinant fragment corresponding to Human ATG3 aa 1-100. Expressed in E. coli.
Database link: Q9NT62 -
ポジティブ・コントロール
- WB: Recombinant human ATG3 protein (amino acids 1-100). ATG3 (AA: 1-100) - hIgGFc transfected HEK-293 cell lysate. HeLa, COS-7, Jurkat, THP-1 and K562 cell lysate. ICC/IF: HeLa and SMMC-7721 cells. IHC-P: Human breast cancer and esophageal cancer tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
7A1D1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab233560の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 36 kDa.
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IHC-P |
1/200 - 1/1000.
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ICC/IF |
1/200 - 1/1000.
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特記事項 |
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WB
1/500 - 1/2000. Predicted molecular weight: 36 kDa. |
IHC-P
1/200 - 1/1000. |
ICC/IF
1/200 - 1/1000. |
ターゲット情報
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機能
E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. -
組織特異性
Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. -
配列類似性
Belongs to the ATG3 family. -
翻訳後修飾
Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 508571 Cow
- Entrez Gene: 64422 Human
- Entrez Gene: 67841 Mouse
- Entrez Gene: 171415 Rat
- Omim: 609606 Human
- SwissProt: Q0VCL3 Cow
- SwissProt: Q9NT62 Human
- SwissProt: Q9CPX6 Mouse
see all -
別名
- 2610016C12Rik antibody
- Apg 3 antibody
- APG3 antibody
see all
画像
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All lanes : Anti-ATG3 antibody [7A1D1] (ab233560) at 1/500 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : THP-1 (Human monocytic leukemia cell line) cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Predicted band size: 36 kDa -
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for ATG3 (green) using ab233560 (1/200 dilution) in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye.
Red: Actin filaments have been labeled with Alexa Fluor®555 phalloidin.
Secondary antibody: Goat anti-Mouse IgG (H+L) DyLight 488.
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Paraffin-embedded human esophageal cancer tissue stained for ATG3 with ab233560 at a 1/200 dilution in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human breast cancer tissue stained for ATG3 with ab233560 at a 1/200 dilution in immunohistochemical analysis. DAB staining.
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SMMC-7721 cells stained for ATG3 (green) using ab233560 (1/200 dilution) in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye.
Red: Actin filaments have been labeled with Alexa Fluor®555 phalloidin.
Secondary antibody: Goat anti-Mouse IgG (H+L) DyLight 488.
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Lane 1 : Anti-ATG3 antibody [7A1D1] (ab233560) at 1/500 dilution
Lane 2 : Anti-ATG3 antibody [7A1D1] (ab233560) at 1/500 dilution (ATG3 (amino acids: 1-100) - hIgGFc transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate)
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : ATG3 (amino acids: 1-100) - hIgGFc transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 36 kDa -
Anti-ATG3 antibody [7A1D1] (ab233560) at 1/500 dilution + Recombinant human ATG3 protein (amino acids 1-100)
Predicted band size: 36 kDa(Expected MW is 37.3 kDa)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab233560 は論文での使用が確認できていません。