Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer (10mM Sodium Citrate, 0.05% T ween 20, pH 6.0)
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Specification
human colon
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: overnight in PBS
Dilution
1/400
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa fluor 594 goat-anti rabbit
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
Dapi was used to stain nuclei.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Steffen Rickelt
Verified customer
投稿 Nov 06 2013