U2OS cells were either treated with Staurosporine (0.5 uM) for 6 hours or left untreated in 6cm dishes. Cells were resuspended using PBS-EDTA and collected in a 15ml tube along with original culture media. Cells were centifuged at 1100rpm for 5 minutes and then resuspended in binding buffer before adding AnnexinV-FITC and PI as in protocol. Cells were incubated for 5 minutes at RT and then analysed.
Additional controls (graphs not shown): no stain, PI only, Annexin V only
Figure legend: U2OS cells were either A. Untreated, or B. Treated with 0.5uM Staurosporine for 6 hours. Percentage of Live (Q3), early apoptotic (Q4) and late apoptotic (Q2) populations are indicated.
Additional controls (graphs not shown): no stain, PI only, Annexin V only
Figure legend: U2OS cells were either A. Untreated, or B. Treated with 0.5uM Staurosporine for 6 hours. Percentage of Live (Q3), early apoptotic (Q4) and late apoptotic (Q2) populations are indicated.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Sarah Sabir
Verified customer
投稿 Nov 26 2013