Application
Western blot
Sample
Human Cell lysate - whole cell (Osteoblast cell line)
Loading amount
10 µg
Specification
Osteoblast cell line
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: TBS/0.1% Tween-20
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/5000
Detection
Detection method
LI-COR Infrared Imaging System
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 37 kDa
Additional data
PubMed ID
20690663: View
Additional Notes
Osteoblast total protein, lane 1,2,3 represent cells at day 5, 10 and 19 of culture
a band of 37 kDa is visible
a band of 37 kDa is visible
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Rodrigo Alves
Verified customer
投稿 Aug 18 2010