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Products:Signal Transduction >> Signaling Pathway >> Lipid Signaling >> PLC
Overview
Product name
Anti-Angiotensin II Type 2 Receptor antibody
Angiotensin II Type 2 Receptor 抗体 (4件) 一覧
Description
Rabbit polyclonal to Angiotensin II Type 2 Receptor
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Rabbit
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Angiotensin II Type 2 Receptor.
(Peptide available as ab90842.)
Positive control
This antibody gave a positive signal in human ovary and testis tissue lysates as well as the following whole cell lysates: HEK293; HeLa.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Blood >> Blood Pressure regulation
Cardiovascular >> Atherosclerosis >> Apoptosis
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> PLC
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Western blot - Angiotensin II Type 2 Receptor antibody (ab78747)
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Immunocytochemistry/ Immunofluorescence - Angiotensin II Type 2 Receptor antibody (ab78747)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Angiotensin II Type 2 Receptor antibody (ab78747)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab78747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 58 kDa (predicted molecular weight: 41 kDa).
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Function
Receptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation.
Tissue specificity
In adult, highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. Very highly expressed in fetal kidney and intestine.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP50052
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AGTR 2 antibody
- AGTR2 antibody
- AGTR2_HUMAN antibody
see all
Database links
- Entrez Gene: 186 Human
- Entrez Gene: 11609 Mouse
- Entrez Gene: 24182 Rat
- Omim: 300034 Human
- SwissProt: P50052 Human
- SwissProt: P35374 Mouse
- SwissProt: Q3US12 Mouse
- SwissProt: P35351 Rat
see all
Anti-Angiotensin II Type 2 Receptor antibody images:
Western blot - Angiotensin II Type 2 Receptor antibody (ab78747)
All lanes : Anti-Angiotensin II Type 2 Receptor antibody (ab78747) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)
Lane 3 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 41 kDa
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
Angiotensin II Type 2 Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Immunocytochemistry/ Immunofluorescence - Angiotensin II Type 2 Receptor antibody (ab78747)
ICC/IF image of ab78747 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78747, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (100 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Angiotensin II Type 2 Receptor antibody (ab78747)
IHC image of Angiotensin II Type 2 Receptor staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78747, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
References for Anti-Angiotensin II Type 2 Receptor antibody (ab78747)
ab78747 has not yet been referenced specifically in any publications.
Publishing research using ab78747? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"