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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Angiotensin II Type 2 Receptor antibody (ab19134)
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Immunocytochemistry/ Immunofluorescence - Angiotensin II Type 2 Receptor antibody (ab19134)
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Product Name
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Angiotensin II Type 2 Receptor antibody
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Angiotensin II Type 2 Receptor 抗体 (3件) 一覧
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Angiotensin II Type 2 Receptor
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Immunogen
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Synthetic peptide: CRKSSSLREMETFVS conjugated to KLH, corresponding to amino acids 349-363 of Rat Angiotensin II Type 2 Receptor.
BLAST 'CRKSSSLREMETFVS' with  or 
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Reacts with
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Hu, Rat
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Predicted to react
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Ms
(Due to sequence homology)
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Tested applications
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ICC/IF, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (see Abreview)
WB: 1/800. Detects a band of approximately 44 kDa. (see Abreview)
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell membrane; Multi-pass membrane protein.
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Research areas
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Cardiovascular >> Blood >> Blood Pressure regulation
Cardiovascular >> Atherosclerosis >> Apoptosis
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> PLC
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Relevance
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Angiotensin II interacts with two types of G-protein coupled membrane receptors, AT-1 (type 1) and AT-2 (type2). AT-2 is a seven transmembrane domain G-protein coupled membrane receptor comprised of 363 aa. It is highly expressed in myometrium, with lower levels in adrenal and fallopian tube in rats. It is also expressed at high levels in fetal kidney and intestine.
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Angiotensin II Type 2 Receptor antibody (ab19134)の別名 |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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2.600 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 Angiotensin II Type 2 Receptor antibody について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for Angiotensin II Type 2 Receptor antibody images, references, products related to ab19134 and other tools.
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Angiotensin II Type 2 Receptor antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Angiotensin II Type 2 Receptor antibody (ab19134)
ab19134 at 1/2000 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum prior to incubation with the antibody for 18 hours. An HRP conjugated horse polyclonal antibody was used as the secondary. This image is courtesy of an Abreview submitted by Miss Laura Palmer
See Abreview
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Immunocytochemistry/ Immunofluorescence - Angiotensin II Type 2 Receptor antibody (ab19134)
ICC/IF image of ab19134 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for Angiotensin II Type 2 Receptor antibody (ab19134)
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This product has been used in: (two most recent references)
Zhang Q et al. FXR-mediated regulation of angiotensin type 2 receptor expression in vascular smooth muscle cells. Cardiovasc Res 77:560-9 (2008). PubMed: 18006431
Portela VM et al. Regulation of angiotensin type 2 receptor in bovine granulosa cells. Endocrinology 149:5004-11 (2008). PubMed: 18583424
See all 3 publication references for this product.
If you publish research using ab19134 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Angiotensin II Type 2 Receptor
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Angiotensin II Type 2 Receptor antibody - more information
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