Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445)

I would be happy if someone would guide me to choose good antibody from your products for anti-Angiotensin Converting Enzyme 1 and 2 for Immunofluorescence and Western applications in Rat tissue.

Since we tried lot of antibody with other sources, it wouldn’t give good result.

ANSWER:

Thank you for your enquiry.

Here is an antibody that has be used in western blotting and IHC on rat samples for ACE 1:
http://www.abcam.com/Angiotensin-Converting-Enzyme-1-antibody-9B9-ab77990.html

Here is an antibody that has be used in western blotting and IHC on rat samples for ACE 2:
http://www.abcam.com/Angiotensin-Converting-Enzyme-2-antibody-EPR4435-2-ab108252.html

I hope this is helpful. Please contact me again if you have any further questions.

Hi, I am going to place the order for this “Anti-Androgen Receptor antibody [AN1-15] (ab2742)”. I need to know what concentration and which buffer would be ideal for this product. Rat Mesentric artery, RIPA protein, 20ug protein, PVDF membrane. Blocked with 5% skimmed milk.

ANSWER:

Thanks for your enquiry. For western blotting, its recommended to use 1ug/ml as the starting concentration for ab2742. This may need to be optimized. For the antibody diluent, you can use TBS/0.5% Tween 20 (TBST). I hope this is helpful. Please let me know if you have any further questions.

  Hi, Thank you very much, its working very well and I got it. Could you please give suggestions about another antibody, ab11734. We are struggling for this antibody working in rat mesenteric artery protein, homogenized with RIPA buffer.

ANSWER:

Glad to hear your experiments are working well. I would be happy to help you troubleshoot your experiments with ab11734. So I can help you in the most efficient manner, please provide some more details regarding the application, sample and sample preparation methods and protocols you are using with this antibody. Thanks for the additional information!

Thanks for your prompt response.

ANSWER:

I'm happy to help. Please don't hesitate to contact me if you have any further questions.

  Hi, I have received ab92445 primary antibody from ABCAM (Not only this product, we use to use lot of antibody from ABCAM). The data sheet does not have the details about primary antibody dilution buffer? I would like to know which buffer I should use either 5% non fat dry milk or 3% BSA or none. Your response will be highly appreciate.

ANSWER:

Thank you for your enquiry.

I recommend using a buffer containing 3% BSA.

I hope this is helpful. Please contact me again if you have any further questions.