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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> Angiopoietin
Overview
Product name
Anti-Angiopoietin 2 antibody
Angiopoietin 2 抗体 (4件) 一覧
Description
Rabbit polyclonal to Angiopoietin 2
Specificity
This antibody is specific for mouse Angiopoietin 2 (mAng-2), and shows a band at 75kD.
Tested applications
ICC/IF, IHC-Fr, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: CNNFRKSVDSTGRRQYQVQNGP, corresponding to amino acids 21-40 of Mouse Angiopoietin 2. (Peptide available as ab9077).
CNNFRKSVDS TGRRQYQVQN GP
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.02 K Phosphate, 0.15M NaCl, 0.1% Sodium Azide, pH7.2
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Angiogenesis >> Growth Factors >> Angiopoietin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/200
IHC-Fr: Use at an assay dependent dilution. (PubMed: 19553662Use at an assay dependent dilution (PMID 19553662).)
WB: 1/500(There is reaction with serum in the cell supernatants, which results in strong background and makes it difficult to see the angiopoietins in cell supernatants. However when precipitated (using soluble Tie2) the signals are very good and strong.)
Target
Function
Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
Sequence similarities
Contains 1 fibrinogen C-terminal domain.
Domain
The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.
Cellular localization
Secreted.
Target information above from: UniProt accessionO15123
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AGPT 2 antibody
- Agpt2 antibody
- ANG 2 antibody
see all
Database links
- Entrez Gene: 285 Human
- Entrez Gene: 11601 Mouse
- Omim: 601922 Human
- SwissProt: O15123 Human
- SwissProt: O35608 Mouse
- Unigene: 583870 Human
- Unigene: 439874 Mouse
Anti-Angiopoietin 2 antibody images:
Western blot - Angiopoietin 2 antibody (ab8452)
Supernatants of mouse angiopoietin-expressing endothelial cells. Soluble Tie 2 was used to precipitate the angiopoietins to reduce background.
Lane 1 - mock
Lane 2 - mouse angiopoietin-2 (clone 2-9) expressing cells
Lane 3 - mouse angiopoietin-1 (clone 1-15) expressing cells
Lane 4 - mouse angiopoietin-1 (clone 1-8) expressing cells
Lane 5 - wt
Marion Scharpfenecker 2002
Immunocytochemistry/ Immunofluorescence - Angiopoietin 2 antibody (ab8452)
ICC/IF image of ab8452 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8452, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Angiopoietin 2 antibody (ab8452)
This product has been referenced in:
- Liu XSet al. Angiopoietin 2 mediates the differentiation and migration of neural progenitor cells in the subventricular zone after stroke. J Biol Chem 284:22680-9 (2009). IHC-Fr; Mouse.Read more (PubMed: 19553662) »
- Siddiqui IAet al. Green tea polyphenol EGCG sensitizes human prostate carcinoma LNCaP cells to TRAIL-mediated apoptosis and synergistically inhibits biomarkers associated with angiogenesis and metastasis. Oncogene 27:2055-63 (2008). WB; Human.Read more (PubMed: 17998943) »
See all 4 publications for this product
Publishing research using ab8452? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"