Application
Western blot
Sample
Human Cell lysate - whole cell (Prostate Cancer cell line)
Loading amount
15 µg
Specification
Prostate Cancer cell line
Gel Running Conditions
Reduced Denaturing (7%)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: RT°C
Other product details
Dilution
1/200
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST+3%BSA
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-mouse IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/200
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 100-110KDa (wt) 70-7 kDa
Additional data
Additional Notes
Upper band at 100-110KD corresponds to the "wild type AR." Lower band 70-75 KDa corresponds to a "truncated AR".
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Víctor Campa
Verified customer
投稿 Dec 30 2009