Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: Ficoll-Hypaque, RBC lysis
Sample buffer: 2% human serum, 0.5 mM EDTA in PBS
Sample buffer: 2% human serum, 0.5 mM EDTA in PBS
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
lymphocytes
Other product details
Dilution
1/100
Incubation time
25 minute(s) · Temperature: 4°C · Diluent: 2% human serum, 0.5 mM EDTA in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 goat anti—rabbit IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Dilution
1/250
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投稿 Aug 03 2012